fotios Papadogiannis


Fotios Papadogiannis MSc
Mobile Phone: 07541678756,+306977195930
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Address: Tollington House,598-602 Holloway Road,
Flat 8, N12 3PG


Education

Sep 2016 – Oct 2018  Masters (MSc) research project in Materials Science and Technology, University of Crete, Greece.
Thesis project title: “Bone tissue engineering with chitosan : gelatin scaffolds seeded with mesenchymal stem cells, MSCs”.

March 2018-Sept 2018 Visiting Scientist at Imperial College London  - Department of Chemical Engineering, Imperial College ,London                       

Sept 2015- July 2016 Bachelor (BSc Hons) research thesis in Materials Science and Technology, University of Crete, Greece.

Thesis title: “Evaluation of the differentiation potential of human bone marrow-derived mesenchymal stem cells (MSCs) using a chitosan-gelatin porous scaffold for bone tissue repair”.
Grade:10/10

Feb 2015 – May 2015  Intern - Haemopoiesis Research Laboratory of the Medical School of  the University of Crete, Greece..Haemopoiesis Research Laboratory of the   Medical School of the University of Crete, Greece.
Grade:9.5/10  

EMPLOYMENT

03/2017-03/2018        Public Umbilical Cord Blood Bank of Crete

                                    Responsible for the information of parents interested to donate the umbilical cord blood of the neonates on issues related to cord blood banking.                      

                                    Supervisors: Prof. H.A. Papadaki, I. Mavroudi, PhD

07/2019-08/2019        Visiting scientist at Anthony Nolan Research Institute

                                    Main techniques:

                                    Isolation of umbilical cord mesenchymal stem cells and umbilical cord blood mesenchymal stem cells and natural killer cells.

                                    Flow cytometry.                      

                                    Supervisor: Dr Diana Hernandez.

09/2019-01/2020         Research Assistant in Haemopoiesis Research Laboratory of the Medical School of  the University of Crete, Greece.

09/2020-now               Associate Researcher in Chemical and Processing Engineering Laboratory of the University of Surrey

04/2021-now               Manufacturing Officer in Virocell Biologics Ltd , London, United Kingdom

PUBLICATIONS

  • Papadogiannis F, Batsali A, Klontzas ME, Karabela M, Georgopoulou A, Mantalaris A, Zafeiropoulos NE, Chatzinikolaidou M, Pontikoglou C. Extracellular matrix of bone marrow mesenchymal stem/stromal cells undergoing osteogenic differentiation in chitosan/gelatin scaffolds: Gene expression profile and mechanical analysis.

Accepted in Biomedical Materials.

  • Georgopoulou A, Papadogiannis F, Batsali A ,Marakis J ,Alpantaki K ,Eliopoulos AG, Pontikoglou C ,Chazinikolaidou M., Chemically crosslinked chitosan/gelatin scaffolds promote osteogenic response: an in vitro an in vivo study. Journal of Materials Science: Materials in Medicine (2018) 29:59
  • Damianaki A, Stagakis E, Mavroudi I, Spanoudakis M, Koutala H, Papadogiannis F, Kanellou P, Pontikoglou C, Papadaki HA., Minor populations of paroxysmal nocturnal hemoglobinuria-type cells in patients with chronic idiopathic neutropenia. Eur J Haematol, 2016 Dec;97(6):538-546.

CONFERENCES-PRESENTATIONS

  • Presented my scientific projects in conferences of 2017 28th Annual Conference of the European Society for Biomaterials in Athens, Greece ,2015 10th Anniversary Conference of the Hellenic Society for Biomaterials, Athens, Greece,2019 Termis EU Meeting Rhodes,Greece., 2021 AlChE Annual Meeting in Boston, MA
  • Conferences attended: 2015 10th Anniversary Conference of the Hellenic Society for Biomaterials, Athens, Greece, 2017 28th Annual Conference of the European Society for Biomaterials in Athens, Greece , 2019 World Advanced Therapies and Regenerative Medicine, Congress and EXPO ,London,UK, 2021 World Advanced Therapies and Regenerative Medicine, London,UK, 2021 AlChE Annual Meeting in Boston, MA

EXPERIMENTAL SKILLS - TRAINING

  • Cell culture of non-adherent cell types: bone marrow-derived human haemopoietic stem cells (HSCs)
  • Cell culture of adherent cell types: bone marrow derived mesenchymal stem cells (MSCs)
  • Flow cytometry (characterization of BM-MSCs with the non-haemopoietic surface antigens CD73, CD90, CD105 and the haemopoietic antigens CD45, CD34 ,CD19, CD14, HLA-DR)
  • In vitro differentiation of MSCs towards adipocytes and osteocytes, Staining of differentiated MSCs (Oil Red O, Alizarin Red, Von Kossa)
  • MTT colorimetric assay (metabolic activity assay)
  • RNA extraction
  • Real time qRT-PCR,
  • Fabrication of porous chitosan-gelatin scaffolds
  • In vitro assessment of MSC behaviour in biomaterials (adhesion, viability, proliferation and osteogenic differentiation)
  • Immunofluorescence on cells(Confocal Laser Scanning Microscopy, endogenous expression of proteins)
  • Cell culture of UCB-MCs in bioreactor
  • Keratinocytes culture
  • Confocal Laser Scanning Microscopy Training by Nikon
  • Cryogens Training
  • Viral Vectors Manufacturing

PERSONAL SKILLS

  • Creative thinking and innovation, g. the development of new ways to approach the therapy of the disease which I currently study.
  • Attention to detail and highly
  • Problem solving, g. re-directed projects successfully into new approaches when they reached a dead-end.
  • Constant self-improvement by staying updated on the latest discoveries on my field of expertise, e.g. constant literature
  • Self-motivated person who maintains excellent
  • Always searching to expand my knowledge even further in new areas.

COMMUNICATION SKILLS

  • Languages Greek – native speaker,English – C2 level,German – B1 level
  • Computer skills Ms Windows, Ms Office (ECDL certified), Graphpad Prism, EndNote
  • Attended weekly meetings of large groups (15+ people) or small groups (7 people or less) during my bachelor, master and Erasmus education position

SCIENTIFIC ASSOCIATION MEMBERSHIPS

2016: Hellenic Society for Biomaterials

2016: European Society for Biomaterials

FUNDING

  • Research funding 1500 € from the Secretariat of Research of the University of Crete for PCR-array reagents for the ECM evaluation of bone marrow-derived mesenchymal stem cell cultures on a chitosan-gelatin scaffold (2018).
  • Research funding 10000 € (I will be research assistant in the project: MSCs culture, Expression of Wnt-induced secreted protein-1, (WISP-1), Fabrication of Biomaterial)

REFEREES

References available on request.